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. 2015 May 7;14(10):1583–1595. doi: 10.1080/15384101.2015.1026486

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Figure 2. — Detergent-free purification of caveolin-rich membrane fractions by sucrose gradient. (A) HEK-293 stably expressing LOX-1-V5 (clone 19) were treated or not with different statins, as indicated, for 3 d. Lysates were subjected to sucrose gradient centrifugation to isolate caveolin-enriched lipid rafts and immunoblotted with anti-V5 (α LOX-1)(left panel) and anti-caveolin (α Cav-1)(right panel). (B) Densitometric measurements of LOX-1 subunit band in fraction 5 derived from control or treated cells with 2.5 μM drug concentration. The data represent the average ± standard deviation (SD) of 3 separate experiments.