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. 2015 May 7;14(10):1583–1595. doi: 10.1080/15384101.2015.1026486

Figure 3.

Figure 3.

Effects of short exposure of statins on LOX-1 mediated ox-LDL binding. (A) COS cells transiently transfected with LOX-1-V5 were incubated with 10 μg/ml DiI-ox-LDL (red fluorescence) for 1 hour at 4°C in the absence (Ctrl) or in the presence of 2 μM lovastatin, fluvastatin or 4 μM atorvastatin. Nuclei are blue stained with Hoechst 33342. Scale bar 20 μm. (B) Histogram shows the percentage of red positive cells with respect of all Hoechst-positive nuclei incubated with 10 μg/ml DiI-ox-LDL without (Ctrl) or with 2 μM lovastatin, atorvastatin, fluvastatin and pravastatin for 1h at 37°C to measure ox-LDL internalization (uptake). (C) Percentage of DiI-ox-LDL red positive cells pre-treated (dashed line) or not (continuous line) with different concentration of lovastatin before the binding assay done in the presence of lovastatin (1h at 4°C). (D) Quantification of DiI-ox-LDL binding by spectrofluorometer. The data represent the mean ± standard deviation calculated from 4 separate experiments. A P value < 0.01 was considered statistically significant.