Figure 8.

Dimers versus monomers ratio. (A) HEK-293 stably expressing LOX-1-V5 (clone #19) were treated or not with lovastatin and fluvastatin for 2 d. Figure shows the protein gel blot analysis of lipid rafts (fractions 4, 5 and 6) purified by sucrose gradient visualized with Mab anti-V5. (B) Western blot of sLOX-1 released in COS transfected culture medium. Equal volumes of medium derived from cells treated or not with different concentration of atorvastatin for 1 hour was TCA precipitated and analyzed by western blot. Samples were run in the absence or in the presence of 20 mM DTT and immunoreactive bands were visualized with Mab anti-V5. (C) Analysis of conditioned medium derived from human umbilical vein endothelial cells treated or not with ox-LDL. Samples were run in the presence of 20 mM DTT and immunoreactive bands visualized with rabbit anti-LOX-1 antibodies. The asterisk indicates a product of LOX-1 degradation, rarely present. D and M refers to dimers and monomers respectively.