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. 2015 Apr 8;14(12):1771–1778. doi: 10.1080/15384101.2015.1026518

Figure 3.

Figure 3.

Knockdown of human ACAP2 reduces 5FU-induced apoptosis in cancer cells. (A, C) HCT116 cells stably expressing shRNAs targeting ACAP2, ACAP3, or a non-targeting control (Ctrl) were treated with 375 μM 5FU for 24 hours prior to analysis of phosphatidylserine externalization (Annexin V) via flow cytometry. Where indicated, cells were pretreated with 2 μM Z-VAD-FMK for 1 hour. (B) A549 cells stably expressing shRNAs targeting ACAP2 or a non-targeting control (Ctrl) were treated and analyzed as in A. All Data shown represent at least 3 independent experiments +/− SEM. P-values were calculated using Student's t-test (unpaired, 2-tailed). (D) Immunoblots showing degree of ACAP2 and ACAP3 knockdown for 2 independent shRNAs.