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. 2014 Dec 8;13(24):3964–3976. doi: 10.4161/15384101.2014.988026

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© 2014 The Author(s). Published with license by Taylor & Francis Group, LLC

© Leandro H Gallo, April N Meyer, Khatereh Motamedchaboki, Katelyn N Nelson, Martin Haas, and Daniel J Donoghue

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — Inhibition of K63-linked ubiquitination Blocks IKKβ Lys171 Signaling. (A) Lysates from HEK293T cells expressing IKKβ WT and K171E were treated with 2 μM and 5 μM NSC697923 for 2 h and immunoblotted for Phospho-IKKα/β (top), IKKβ (middle panel) and β-tubulin (bottom). (B) Duplicate samples from (A) were immunoblotted for Phospho-STAT3 (Tyr705) (top) and STAT3 (bottom). (C) Lysates from HEK293T cells expressing IKKβ K171E treated with +/− 5 μM NSC697923 for 2 h and +/− 10 ng/ml IL-6 for 10 min were immunoblotted as in (B). (D) Lysates from HEK293T cells expressing IKKβ Lys to Arg mutants were immunoblotted as in (B).