Figure 2.

Mad2 and BubR1 regulate cell proliferation. (A) The MKN45 and ST2957 cell lines were transduced with lentivirus expressing sh-MAD2L1 and sh-BUB1B. The graphs show the proliferation rate measured every 24 h up to 7 d after infection, measured by crystal violet method. Data were calculated relative to the staining obtained on the first day. The experiments were done in quadruplicate at least 3 times. (B) Clonogenic assay. The graph represents the colony counting average at day 14, in 3 different areas per well. The experiment was performed in duplicate. (C) Analysis of the accumulated number of duplications over time (PDLs) in the MKN45 cell line and its transduced sh-BUB1R and sh-Mad2 cell lines. Each condition was done in duplicate.