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. 2014 Nov 18;14(1):157–166. doi: 10.4161/15384101.2014.986392

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Figure 3. — B55α association with Plk1. (A) The pull-down assay was performed in Xenopus egg extracts as described in Materials and Methods using purified B55α and other PP2A subunits. Immunoblotting of Plk1 was shown. (B) Immunoprecipitation was performed in in Xenopus egg extracts as described in Materials and Methods using Plk1 antibody. Immunoblotting of B55α and Plk1 was shown. (C) Immunoprecipitation was performed in in Xenopus egg extracts using B55α antibody. Immunoblotting of B55α and Plk1 was shown. (D) N-terminal (aa 1–380) or C-terminal (aa 380–598) Xenopus Plk1 was expressed, purified, and used for pull down assays, as described in Materials and Methods. Input and pull-down products were analyzed by immunoblotting for B55α, PP2A and GST.