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. 2014 Nov 18;14(1):157–166. doi: 10.4161/15384101.2014.986392

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Figure 6. — B55α regulates DNA damage checkpoint recovery through Plk1. (A) Checkpoint recovery was performed in Xenopus egg extracts as described in Materials and Methods, with or without supplementation of B55α. Extract samples were analyzed at the indicated time points for Plk1 Thr-210, Chk1 Ser-317, Cdc25 Ser-216, and Cdc27. (B) Checkpoint recovery was performed in Xenopus egg extracts with supplementation of B55α and Plk1 as indicated. Extract samples were analyzed at the indicated time points for Chk1 Ser-317 and Cdc27. (C) PP2A IP, or control beads IP, was performed in the control interphase extract (ctr), or recovery extract at time 0 (0). The input extracts and the IP products were analyzed by immunoblotting for Plk1 and PP2A. (D) MBP-B55α pull-down was performed in the control interphase extract (ctr), or recovery extract at the indicated time points. The pull-down levels of PP2A and MBP are shown. The control pull-down was performed using amylose beads.