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. 2015 Feb 19;14(8):1188–1196. doi: 10.1080/15384101.2015.1012875

Figure 1.

Figure 1.

Dynamics of conversion of MEFs into neurons by exogenous BAM expression. (A) Experimental design. MEFs were plated at day -2, transduced with BAM+RT viruses at day -1 and cultured in the presence of DOX starting from day 0 until day 5–19. (B) Immunostaining with Tuj1 (yellow) and NF200 (red) antibodies in the targeted cells 5–19 d after viral transduction. (C) Same as B for neuronal markers Tuj1 (yellow) and MAP2 (red) antibodies. (D) Immunostaining with NF200 (red) and collagen-1 (green) antibodies of the targeted cells 19 d after viral transduction. Cell nuclei (DAPI) are shown in blue.