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. 2015 Feb 6;14(3):449–458. doi: 10.4161/15384101.2014.977081

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — Increasing levels of hMDMX^p60 induces oscillation in hMDM2 expression levels in H1299 cells. (A) Expression of a fixed amount of hmdm2 and increasing levels of either (a) hMDMX^p60 alone or (b) together with a fixed amount of hmdmx^fl, or (c) increasing levels of both isoforms. The hmdm2(C464A) mutant (d) is E3 ligase dead and is not affected by hMDMX^p60 (Fig. S4A and B). (B) In vitro autoubiquitination of recombinant hMDM2 in the presence of increasing levels of hMDMX^p60. Data shows one representative experiment out of 3 (Fig. S5A and B).