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. 2015 Jan 3;13(24):3867–3877. doi: 10.4161/15384101.2014.972882

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© 2014 The Author(s). Published with license by Taylor & Francis Group, LLC

© Yann Thomas, Marion Peter, Francisca Mechali, Jean-Marie Blanchard, Olivier Coux, and Véronique Baldin

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — CDC25B phosphatase activity is required to induce multipolar spindles in cells. HeLa cells were transiently transfected with pmCherry-CDC25B (-Wt, -C/S or -DDA) or pmCherry expressing vectors. Fourteen hours post-transfection, cells expressing mCherry-tagged CDC25B-Wt, CDC25B-C/S, CDC25B-DDA, or mCherry alone were imaged by time-lapse microscopy for 24 hours. The number of poles per mitotic spindle was counted. Data represent the percentage of cells with 2 or more than 2 poles and values are the mean ± SD of 3 independent experiments. Total number of counted cells: mCherry, n = 161; mCherry-CDC25B-Wt, n = 106; mCherry-CDC25B-C/S, n = 126; mCherry-CDC25B-DDA, n = 144.