Table 2.

Positions in ZEBOV that are likely associated with the loss of human pathogenicity by RESTV

Name	UniProt ID	Length	P-value	Mutations associated with the loss of human pathogenicity
GP	Q05320	676	0.457	F31I, Q44K, V45A, E156N, S196A, L199A, S210T, Y261R, T269S, T283P, S307H, T335P, E337T, H339N, E345T, H354L, E359T, A361E, A427M, G488K, R498K, R500K, N514D, D607S, K622E, I627K, Q638H, D642L, W644L, T659I
L	Q05318	2212	0.690	V66T, E93T, Q109H, N120A, V128T, E130I, F132T, L146V, L179F, N201T, T202I, A221S, Q223L, H227Q, V229L, P262V, V263D, S274L, L283V, Y312F, A326S, T330D, S343Y, E350D, T361S, L365F, I402N, Q447H, P450S, D465N, R654H, E689S, S847A, S868A, F896Y, L925F, A954S, S995T, T1024N, R1073K, A1119S, Q1149P, S1154L, P1163A, K1171D, D1189S, A1214S, R1217K, D1237E, Q1253N, Y1322L, R1354K, T1366A, I1408M, S1436N, K1461Q, S1473C, L1488Y, S1506A, A1538S, V1562L, E1564S, T1571K, Q1608I, H1619L, L1624Y, C1628S, D1744G, E1752P, S1769G, Q1782L, R1792H, W1822L, V1850T, R1916N, K1938Q, E1941R, V1955Y, Q2024G, P2038V, S2077T, K2078G, R2079L, E2098D, Q2105L, Q2108E, Y2131F, L2157V, R2168H, R2175K, L2177F, M2186L, L2203F
NP	P18272	739	0.587	R4G, T15G, S30T, R39K, I52M, R105K, M137L, F212Y, K274R, S279A, K373R, K374R, A411L, K416N, Y421Q, D426E, D435N, Q442L, D443E, T453I, V458A, D492E, Q507S, S511I, N551R, T563S, E633L, S647K, A705R, T714Y, D716N
VP24	Q05322	251	0.932	T131S, N132T, M136L, Q139R, T226A, S248L
VP30	Q05323	288	0.010	G20P, V25S, Y39R, T52N, V53L, T63I, E93D, T96N, R98H, K107R, S111I, L116S, N117Q, A120S, Q135S, T150I, Q157R, R196H, E205D, R262A, S268Q
VP35	Q05127	340	0.019	T5L, L25T, S26T, E48D, D76E, C79Y, N80V, E85K, S92M, V97T, Q98S, S106A, A154S, T159V, E160D, G167K, S174A, I258T, E269D, A290V, A291P, V314A, Q329K
VP40	Q05128	326	0.786	M14N, T46V, P85T, A128I, G201N, F209L, Q245P, H269Q, T277Q, V323H, E325D

P-value: binomial test for enrichment of residues that may be associated with RESTV's loss of human pathogenicity in each protein.