Skip to main content
. 2014 Oct 30;13(19):3076–3082. doi: 10.4161/15384101.2014.949144

Figure 2.

Figure 2.

LAb T-ALLs Lack Clonal Chromosome t(12;14) Translocations that Delete Bc111b. (A) SKY images of metaphase spreads prepared from LAb T-ALLs #211 or #321. The clonal chromosome t(12;14) and t(14;12) translocations of LAb T-ALL #211 and the clonal chromosome t(14;X) and t(14;15) translocations of LAb T-ALL #321 are circled. (B) Schematics (top) and PCR analyses (bottom) of Bcl11bWT, Bcl11bflox, and Bcl11bΔ alleles. Top, shown for each allele are locations of the Bcl11b exons, loxP sites, and PCR primers. The no. 1 primer set amplifies distinct bands from Bcl11bWT and Bcl11bflox alleles, while the no. 2 primer set amplifies a band from only Bcl11bΔ alleles. Bottom, Images of PCR no. 1 or no. 2 products from genomic DNA of the indicated LAb T-ALLs, LAb thymocytes, LAb kidney, or WT kidney. The images were cropped from a larger blot.