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. 2015 Dec 1;490:66–72. doi: 10.1016/j.ab.2015.08.025

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig.1 — Comparison of enzyme-linked immunosorbent assay (ELISA) (A) and click chemistry armed ELISA (click–ELISA) (B). (A) Analyte is captured on the plate and detected with primary antibody, followed by secondary antibody-HRP conjugate. Colorimetric readout is proportional to bound analyte. (B) Biotinylated substrate modified with YnC₁₅ alkyne tag is captured on a streptavidin-coated plate and functionalized with FLAG-tag via copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). FLAG-tag is detected by α-FLAG-HRP conjugate. Colorimetric readout is proportional to alkyne-tagged substrate.