Table 1.
Acylation activity of lysate fractions and P100(sol) fractions solubilized in the presence of different detergents.
| Fraction | OD450 | Relative activity (%) |
|---|---|---|
| P100 | 0.24 ± 0.03 | 27 ± 1 |
| S100 | 0.05 ± 0.00 | −2 ± 1 |
| P100(Triton) | 0.07 ± 0.01 | 1 ± 1 |
| P100(OTG) | 0.21 ± 0.07 | 18 ± 7 |
| P100(CHAPS) | 0.37 ± 0.09 | 41 ± 6 |
| P100(DDM) | 0.76 ± 0.14 | 100 |
| DDM buffer | 0.06 ± 0.01 | 0 |
Relative activity is background corrected to DDM buffer signal and reported as percentage relative to P100(DDM) signal. Detergent abbreviations are as described in Fig. 2. Values represent means ± standard errors. Assays were performed in duplicate, n = 2.