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Vmax and apparent Km values for YnC15-CoA and Shh(1–11) substrates assessed by click–ELISA at 3.0 μM Shh(1–11) and 5.0 μM YnC15-CoA, respectively.
| Substrate | Vmax (pmol/min) | Apparent Km (μM) |
|---|---|---|
| Shh(1–11) | 1.08 ± 0.06 | 1.37 ± 0.25 |
| YnC15-CoA | 0.87 ± 0.06 | 0.15 ± 0.05 |
Values represent means ± standard errors. Assays were performed in duplicate: Shh(1–11), n = 3; YnC15-CoA, n = 4.
