Figure 3.

Mitochondria-derived ROS activate the ATM kinase. (A) CAFs were treated with or without NAC (1 mM) for 24 h. Immunoblotting analyses were done with the indicated antibodies. (B) CAFs were treated with PDTC (20 μM), DPI (10 μM), Oxypurinol (100 μM), NADPH (1 mM), Chloramphenicol (300 μM), and Rotenone (1 μM) for 2 h. The intracellular ROS levels were measured in cultures (*, P < 0.05, CAFs treated with inhibitor vs control CAFs). (C) CAFs were treated as in B. Western blots was done with the indicated antibodies (*, P < 0.05, CAFs treated with inhibitor vs control CAFs).