Figure 7.

(See previous page). Oxidized ATM enhances the CAFs proliferation through activating key proliferation-related signaling pathways. (A) CAFs were treated with KU60019 (5 μM) for the indicated time points. Immunoblotting analyses were performed with the indicated antibodies. Total histone 3 functions as a loading control for nuclear proteins. (B) CAFs were treated separately or jointly with LY294002 (20 μM), U0126 (25 μM), and XAV939 (10 μM) for the designed time. Their growth curves were determined by MTT assay (*, P < 0.05, untreated CAFs vs treated CAFs with indicated inhibitor(s)). (C) Photomicrographs of CAFs treated with or without inhibitor(s) as in B indicated for 2 d. Scale bars, 50 μm. (D) Flow cytometry analyses of CAFs treated with indicated inhibitor(s) for 24 h as in B. (E) CAFs were treated separately or jointly with LY294002 (20 μM), U0126 (25 μM), and XAV939 (10 μM) for 24 h. Immunoblotting analysis was done with the indicated antibodies. Total histone 3 functions as a loading control for nuclear proteins. (F) CAFs were treated separately or jointly with LY294002 (20 μM), U0126 (25 μM), and XAV939 (10 μM) for 24 h. The levels of p-RB, c-Myc, p21^Cip1, and p16^Ink4a were analyzed by western blot. (G) CAFs were treated with KU60019 (5 μM) for the indicated time. Immunoblotting analysis was performed with the indicated antibodies.