Figure 1.

SEC analysis of AMBRA1, BECLIN 1 and PP2A-C complex. (A) Protein extracts from HEK293 cells were separated into different fractions and the presence of AMBRA1, BECLIN 1 and PP2A-C was assessed by Western Blot, using anti-AMBRA1, anti-BECLIN 1 and anti-PP2A-C antibodies. (B) Protein extracts, collected from HEK293 cells upon autophagy induction (EBSS treatment; starvation), were treated as in (A). Of note, variations of AMBRA1 and PP2A-C profiles can be observed upon autophagy induction [(B), fractions 23-27], with respect to the control condition (A). Although the meaning of these observations needs further investigation, upon starvation changes in the composition of the macromolecular complex can be hypothesized, most likely due ambra1, BECLIN 1 and PP2A-C interactors joining or leaving the complex.