Figure 1.

Impact of PTCH1b 5'UTR size and CGG-repeat number in gene reporter assays. pGL3-Promoter-derived constructs containing PTCH1b 5'UTRs (188 or 300 nucleotides) fused to the Firefly luciferase (Fluc) were transiently transfected in HCT116 ^p53+/+ (A), MCF7 (B) and HEK 293T cells (C). Presented are the average fold inductions relative to the empty vector and standard deviations of at least 3 biological replicates. The luciferase values are expressed relative to the value obtained with an empty control vector. The same constructs were used to measure relative Fluc mRNA transcript levels in transiently transfected HCT116 ^p53+/+ (D), MCF7 (E) and HEK 293T cells (F). Presented is the average Fluc mRNA expression normalized for the average plasmid copy number and for relative cDNA synthesis efficiency as revealed by the amplification of GAPDH and B2M mRNAs. For each UTR size type, results were compared to the corresponding reference allele with (CGG)₇; *P < 0.05