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. 2015 Mar 31;12(3):290–304. doi: 10.1080/15476286.2015.1008929

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Figure 5. — An internal ribosome entry site (IRES) motif maps in the 3' end of the PTCH1b 5'UTR. (A) Putative PTCH1b IRES motif cloned into pRuF-type plasmid is not sufficient to obtain Firefly luciferase (Fluc) activity observed with the complete PTCH1b 5'UTR in MCF7 cells, while the remaining part of PTCH1b 5'UTRs (188ΔIRES and 300ΔIRES) retains certain levels of IRES activity. (B) Similar results were obtained in HEK 293T cells. (C) The ratios between Fluc and Renilla luciferase (Rluc) mRNA for remodeled pRuF PTCH1b 5'UTR plasmids in transfected MCF7 cells didn't deviate from 1.0. (D) The same results were obtained in HEK 293T cells. Results are presented as described in Figure 4.