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Hypoxia enhances PTCH1b 5'UTR mediated translation. MCF7 and HEK 293T cells transiently transfected with the different pRuF reporter constructs were grown in normoxic (dark gray) or severe hypoxic (light gray) conditions for 16 hours. Luciferase assays were performed as described in Materials and Methods and shown as those presented in Figure 5.
