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. 2015 Mar 31;12(3):255–267. doi: 10.1080/15476286.2015.1017221

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© Wen-An Pan, Hsin-Yue Tsai, Shun-Chang Wang, Michael Hsiao, Pei-Yu Wu, and Ming-Daw Tsai

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Silencing of NIFK induces reversible p53 dependent G1 arrest. (A) Cellular proliferation of U2OS cells transfected with siNIFK. (B) Flow cytometry analyses of asynchronous and G2/M synchronous U2OS cells transfected with siNIFK. (C) Same as B, with indicated siRNA alone or in combination. The quantifications of 2 repeats are also shown in the right panel. (D) Western blot analysis of the expressions of NIFK, p53, and p21 in asynchronous U2OS cells transfected with indicated siRNA.