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. 2015 Mar 31;12(3):255–267. doi: 10.1080/15476286.2015.1017221

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© Wen-An Pan, Hsin-Yue Tsai, Shun-Chang Wang, Michael Hsiao, Pei-Yu Wu, and Ming-Daw Tsai

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — NIFK is required for G1 progression by participating in rRNA processing. (A) Flow cytometry analysis of G2/M synchronous U2OS cells transfected with indicated siRNAs (left panel), and Western blot analysis of indicated proteins of asynchronous cells (right panel). (B) Immunofluorescent staining of U2OS cells showing subnucleolar localization of NIFK (green), Ki67 (red), fibrillarin (red), and nuclei (blue). (C) ³²P-orthophosphate based pulse-chase analysis showing the kinetics of nascent rRNA synthesis in U2OS cells transfected with siNIFK. ³²P labeled RNAs are separated by 1% agarose-formaldehyde gel and visualized by autoradiography (upper panel). The total RNA is shown by ethidium bromide (EtBr) staining (lower panel). (D) The same as (C) except the RNAs were separated by a 10% polyacrylamide-7 M urea gel. The total RNA is shown by ethidium bromide (EtBr) staining (lower panel and full view in Fig. S2D).