Figure 3.

Bax/Bak are needed for SIGRUNB. GN-DKO MEFs were treated with 25 μM cisplatin (Cis) or 1 μM camptothecin (Camp) or left untreated. After 18 h, Hoechst 33342 was added to the culture medium and GFP-nucleolin and Hoechst fluorescence were monitored as described in Figure 1. The results shown (still images from time lapse imaging) are from the same field visualized separately for detection of GFP-nucleolin (upper panels) and Hoechst 33342 (lower panels). Indicated time points (min:s) are expressed relative to the first image selected for presentation (t = 00:00) which corresponds to the image captured at the beginning of the recording. Bar = 10 μm. The images shown are from representative experiments from 2, 3 and 2 independent experiments for untreated, Cis and Camp treated cells, respectively.