Table 2.

Composition of experimental diets

Ingredient	Safflower oil (g)	Fish oil (g)
Casein (vitamin free)	395	395
Sucrose (Teklad #160482)	454	454
Corn starch (Teklad #160170)	908	908
Safflower oil	202	26.26
Fish oil	0	175.7
Vitamin mix (Teklad #40060)*1	20	20
Mineral mix (AIN 93)*2	100	100
Choline chloride (Sigma C1879)	2	2
Methionine (Sigma M9500)	6	6
Cellulose (Teklad #160390)	100	100
SeO2 (Sigma S9379)	0.168mg (200ml)	0.168mg (200ml)
MnCl2 (Fisher M87)	0.599	0.599
Total	2187	2187
Analysis (% by energy)	Experimental Diets	Chow*3
Protein	17.8	24.6
Carbohydrate	61.6	62.2
Fat	20.6	13.2

^*1Vitamin mix: 20,000 IU retinyl palmitate, 2000 IU cholecalciferol, 100 IU α-tocopherol acetate, 5 mg menadione, 5 mg thiamine-HCL, 8 mg riboflavin, 40 mg pyridoxine-HCL, 40 mg niacin, 40 mg panthothenic acid, 2000 mg choline, 100 mg myoinositol, 100 mg para-aminobenzoic acid, 0.4 mg biotin, 2 mg folic acid, and 30 mg cyanocobalamine

^*2Mineral mix:1.1 g calcium carbonate, 36.8 g calcium phosphate, 0.1 g citric acid, 23 mg cupric citrate 0.5 H₂O, 1.3 g magnesium oxide, 418 mg manganese citrate, 0.5 mg potassium iodide, 3.4 g potassium sulfate, 1.5 g sodium chloride, 1.1 g sodium phosphate, 66.5 mg zinc citrate 2 H₂O with an additional 78 mg Mn²⁺, 60 μg Se²⁺

^*3The chow diet (PicoLab Rodent Diet 5053; LabDiets, St. Louis, MO) used as a ‘control’ was semi-synthetic in origin with natural ingredients. On average, the diet composed of 20% w/w protein, 5.6% w/w fats [2.1linoleic, 0.29 α linolenic, 0.93 saturated and 0.99 monounsaturated fats] and 53% carbohydrates [starch 34, glucose0.19, sucrose3.18, fructose 0.23, lactose1.34] and 4.7% fiber. Minerals were provided by Ash (6.1%w/w). Minerals and vitamins were added according to the AIN76A guidelines. Detailed compositions of vitamins and minerals are found in http://www.labdiet.com/cs/groups/lolweb/@labdiet/documents/web_content/mdrf/mdi4/∼edisp/ducm04_028436.pdf