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. 2015 Apr 18;12(7):728–741. doi: 10.1080/15476286.2015.1040978

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

© Benjamin P Towler, Christopher I Jones, Sandra C Viegas, Patricia Apura, Joseph A Waldron, Sarah K Smalley, Cecilia M Arraiano, and Sarah F Newbury

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Knockdown of Dis3 in the wing imaginal disc results in severe developmental phenotypes. Knockdown of Dis3 in the wing pouch of the wing imaginal disc results in a “no wing” phenotype at 100% penetrance (Dis3^KD) (B) when compared to a UAS-Dis3^RNAi parental control male fly (Dis3^WT) (A). (C) Knockdown of Dis3 protein in nub-GAL4/+; UAS-Dis3^RNAi/+ wing imaginal discs by Western blotting. Dis3^WT genotype: ; nub-Gal4;. (C) Dis3 expression is knocked down to 20% of parental controls in wing imaginal discs when UAS-Dis3^RNAi is driven by nub-GAL4. 'Dis3^WT' includes parental genotypes ; nub-Gal4 ; and ;; UAS-Dis3^RNAi. n ≥ 3, p = 0 .0009, error bars show standard error. (D and E) nub-GAL4/+; UAS-Dis3^RNAi/+ flies also show a severe lack of haltere development (E) (100% penetrance) compared to parental control flies (D). (F) Dis3 knockdown progeny (nub-GAL4/+ ; UAS-Dis3^RNAi/+) are delayed in larval development by 40 hours (red) when compared to parental controls (black – UAS-Dis3^RNAi and nub-GAL4) (n ≥ 23 ).