Figure 1.

Relatively high expression of the MEF2Cγ- transcript was observed in mature brown adipocytes (BAs). (A) Schematic of the alternative spliced γ region (diagonal bar) in MEF2C exon 10. Total RNA isolated from the (B) wild-type and RBM4^−/− interscapular brown adipose tissues (iBATs), (C) wild-type and RBM4^−/− iBAs cultured in growth medium (GM) and differentiating medium (DM) was subjected to an RT-PCR using a specific primer set against MEF2C (Table S1). The bar graph shows the relative ratio of MEF2Cγ- over total MEF2C transcripts using TotalLab Quant Software (*p < 0.05; **p < 0.01; ***p < 0.005).