Figure 4.

Overexpressed MEF2C variants exerted opposite effects on the differentiation of BAs. (A) C3H10T1/2 cells were mock-transfected or transfected with FLAG-MEF2C variant-expressing plasmids. Total proteins were extracted and subjected to an immunoblotting assay using the indicated antibodies. Total RNAs were subjected to an RT-PCR using specific primer sets against BA-specific factors. (B) Total RNAs extracted from the proliferating (in growth medium; GM) or differentiating (in differentiation medium; DM) cells were subjected to a qRT-PCR using specific primer sets (Table S2). (C) The mock vector or FLAG-MEF2C variant vector-transfected C3H10T1/2 cells were cultured in GM for 48 h and then subjected to oil-red-O staining. The bar graph shows the spectrophotometric analysis of oil-red-O optical densities (ODs) at 550 nm after being extracted from stained cells in the previous panel (*p < 0.05; **p < 0.01; ***p < 0.005).