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. 2015 Sep 20;12(10):805–810. doi: 10.7150/ijms.12429

Figure 4.

Figure 4

Expression of apoptosis-related proteins. A:NB4 cells were cultured with 0-40mM LiCl for 24h,B: NB4 cells were treated with 20mM LiCl/NaCl for 24h.Whole-cell extracts were then prepared and analyzed by western blot.C-G:detection of Akt1, β-catenin, p-GSK-3β, GSK-3β and c-Myc by western blot, β-actin was served as a control. Compared with the control, the expression of Akt1 was inhibited by LiCl in a dose-dependent manner, the expression of c-Myc decreased significantly, and that of β-catnin increased markly. The level of p-GSK-3β(S9) protein was increased remarkably, and the expression of GSK-3β had no significant differences. Data was expressed as means ± SD.*P<0.05 (lane 1: NB4 cells treated with PBS; lane 2: NB4 cells treated with NaCl 20mM; lane 3: NB4 cells treated with LiCl 20mM).