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. 2015 Jan 20;6(1):40–54. doi: 10.1080/19491034.2015.1004260

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

© Michal Schwartz, Anna Travesa, Steven W Martell, and Douglass J Forbes

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — Recruitment of nucleoporins from other NPC subcomplexes to the LacO array. Immunofluorescence microscopy of U2OS 2–6–3 cells transiently transfected with either LacI-CFP-Nup214 (A) or LacI-CFP-Pom121 (B, C), then stained with antibody against LacI or visualized by CFP fluorescence (left panel). The endogenous nucleoporins Nup133 (A), Nup62 (B), or Nup93 (C) were detected with specific antibodies (middle panel). Chromatin is visualized by DAPI staining. The smaller insets show a magnified image of the LacO array in each image. The right hand panels are a merge of the previous 2 images with DAPI staining. All three cases show examples of positive recruitment of an endogenous nucleoporin from a different subcomplex than that of the LacI-CFP-NupX. Full results are summarized in Table 1.