Figure 5.

Enhanced expression of Nup153 alters nuclear organization. HeLa cells were transiently transfected with GFP-Nup153 and siRNAs against Nup153, respectively, and prepared for thin-sectioning EM 48 hours after transfection. Enhanced expression of Nup153 coincided with membranous structures in the cytoplasm (arrow in B), abnormally shaped nuclei (B), an increase in heterochromatin in the nucleoplasm (B, black arrowheads in C) and along the nuclear envelope (B) as well as to segregation of nucleoli and their association with the nuclear envelope (C). Nuclei from control cells and Nup153-depleted cells did not differ from each other. White arrowheads in (C) indicate section artifacts. Scale bars, 2 μm.