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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Methods. 2015 Apr 20;87:83–95. doi: 10.1016/j.ymeth.2015.04.014

Figure 5.

Figure 5

Comparison of the % total individual A) diglyceride (DG) and B) cholesterol ester (CE) molecular species in LIM1215 cells (dark bars) and LIM1215 secreted exosomes (white bars) detected as ammonium adducts by positive ionization mode high resolution / accurate mass spectrometry from the data in Figure 1. Only the most abundant lipids are shown for clarity. The inset to panel A shows the ratio of the total lipid ion abundances (normalized to protein) of DG species that contain at least one unsaturated fatty acid to species containing only saturated fatty acids. The inset to panel B shows the ratio of the total lipid ion abundances (normalized to protein) of CE species that contain at least one long chain fatty acid (≥ 20 carbons) to species that contain only short chain fatty acids, and the ratio of the abundances of species containing at least 1 unsaturated fatty acid to species containing only saturated fatty acids. Error bars show the standard deviations determined by triplicate measurement. * P < 0.05.