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. 2014 Nov 26;5(6):729–736. doi: 10.4161/19490976.2014.972223

Figure 3.

Figure 3.

A.IL-8 release by T84 cells upon infection with intestinal bacteria. T84 cells were infected with invasive S. flexneri, Commensal E. coli HS, S. marcescens, or K. oxytoca for 3 hour and basolateral supernatants collected for assay of pro-inflammatory cytokine IL-8 by ELISA as described under materials and methods. Uninfected cells were used as negative control. Data are means ± standard deviation of the means from triplicate readings of 3 independent experiments. ***S. marcescens was significantly different at P < 0 .0001 from each of the other bacteria tested. 3B: IL-8 release by T84 cells upon infection with different strains of S. marcescens. T84 cells were infected with different strains of S. marcescens and IL-8 released from the cells determined as described under Figure 3A. Data are means ± standard deviation of the means from triplicate readings of 3 independent experiments. ***, (P < 0 .0001) and **, (P < 0 .001).