Table 1.

Barcoded sequencing primers

Primer name	5’ to 3’ sequence (titanium adaptor (– barcode) – primer sequence)
27F-DegS-1	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCAACC – GTTYGATYMTGGCTCAG
27F-DegS-2	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCAAGG – GTTYGATYMTGGCTCAG
27F-DegS-3	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCATCG – GTTYGATYMTGGCTCAG
27F-DegS-4	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCATGC – GTTYGATYMTGGCTCAG
27F-DegS-5	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCGCAT – GTTYGATYMTGGCTCAG
27F-DegS-6	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCGCTA – GTTYGATYMTGGCTCAG
27F-DegS-7	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCGGAA – GTTYGATYMTGGCTCAG
27F-DegS-8	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCGGTT – GTTYGATYMTGGCTCAG
27F-DegS-9	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCTACG – GTTYGATYMTGGCTCAG
27F-DegS-10	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCTAGC – GTTYGATYMTGGCTCAG
27F-DegS-11	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCTTCC – GTTYGATYMTGGCTCAG
27F-DegS-12	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACCTTGG – GTTYGATYMTGGCTCAG
27F-DegS-13	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACGAACG – GTTYGATYMTGGCTCAG
27F-DegS-14	CCATCTCATCCCTGCGTGTCTCCGACTCAG – AACGAAGC – GTTYGATYMTGGCTCAG
titAdapB338R-I	CCTATCCCCTGTGTGCCTTGGCAGTCTCAG – GCWGCCTCCCGTAGGAGT
titAdapB338R-II	CCTATCCCCTGTGTGCCTTGGCAGTCTCAG – GCWGCCACCCGTAGGTGT