Figure 1.
Chromatin fragmentation by sonication or digestion with micrococcal nuclease. (A, B) Chromatin fragmentation patterns after (A) Bioruptor® sonication and (B) MNase digestion (0.37 U/106 cells) followed by a brief sonication pulse as described in Materials and Methods. (C) Western blot analysis of lamin A/C in chromatin and pellet fractions after sonication- or MNase-based fragmentation. Total, cell lysate before fragmentation. (D) Western blot of lamin A/C immunoprecipitated (IP) from sonicated or MNase-digested chromatin. Total, cell lysate; input, chromatin before immunoprecipitation; beads-only were used as negative controls.