Figure 4.

Mutational screening of loop A, single-stranded stretch, and terminator loop in LhrC. Labeled LhrC4 and several mutant derivatives were tested for their ability to bind oppA RNA (4, 20 and 100 fold excess relative to labeled LhrC4). In the LhrC4 sketches, the mutated regions are indicated in black (thick line). LhrC4: wild type LhrC4. LhrC4_mut_4: mutation in loop A. LhrC4_mut_7: mutation in loop A, single-stranded stretch, and terminator loop. LhrC4_mut_2: mutation in single-stranded stretch. LhrC4_mut_3: mutation in terminator loop. LhrC_mut_5: mutation in single-stranded stretch and terminator loop. The fraction of unbound LhrC4 is shown below each lane.