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. 2015 Mar 11;12(4):457–477. doi: 10.1080/15476286.2015.1014288

Figure 5.

Figure 5.

p53 and NFκB p65/RelA transcriptionally regulate the expression of miR-100, −125b, −146a, −150 and −221 (A) Schematic representation of miRNAs which are regulated by p53 only (miR-34a, −145, −199a-5p), NFκB p65/RelA only (miR-15a, −199a-3p, −148a, −135a, −182, −214) and regulated by both p53 and NFκB p65/RelA (miR-15b, −100, −125b, −146a, −150, −221) in both mouse striatal STHdhQ7/HdhQ7 cells and human cervical carcinoma HeLa cells; RT-PCR showing (B) Expression of pri-miR-15b, pri-miR-100, pri-miR-125b, pri-miR-146a, pri-miR-150 and pri-miR-221 in HeLa cells; (C) Decrease in the expression of the pri-miRNAs in presence of overexpressed p53-CFP; data are mean ± SD (n = 3); *P ≤ 0.05; ** P ≤ 0.01 compared to control. (D) Increase in the expression of the pri-miRNAs in presence of over expressed NFκB p65/RelA in HeLa cells; data are mean ± SD (n = 3); *P ≤ 0.05; ** P ≤ 0.01 compared to control. GAPDH mRNA level is taken as endogenous control in each case. Error bars represent standard deviation, * represents statistical significance.