Table 1.
Relationship of DNA damage with lymphatic and neural invasion in ICC patients
| Immuno- histological grading |
8-oxodG
Number of
patients (%) |
8-Nitroguanine
Number of
patients (%) |
HIF-1α
Number of
patients (%) |
Number (%)
of patients
with
lymphatic
invasion (8-oxodG) |
Number (%)
of patients
with
lymphatic
invasion (8-nitroguanine) |
Number (%) of
patients with
neural
invasion
(8-oxodG) |
Number (%)
of patients
with
neural
invasion (8-nitroguanine) |
||
| Cancerous | Non-cancer | Cancerous | Non-cancer | Cancerous | Cancerous | Cancerous | Non-cancer | Non-cancer | |
| ICC (37) | |||||||||
| 0 | - | 5 (13.51) | 11 (29.73) | 17 (46) | - | - | 9/11 (81.82) | 1/5 (20) | 8/17 (47.1) |
| + | 11 (29.73) | 17 (45.94) | 13 (35.14) | 19 (51.4) | 10 (27.03) | 6/11 (54.55) | 10/13 (76.92) | 14/17 (82.4) | 16/19 (84.2) |
| ++ | 11 (29.73) | 10 (27.03) | 8 (21.62) | 1 (2.70) | 18 (48.65) | 10/11 (90.91) | 6/8 (75) | 5/10 (50) | 0/1 (0) |
| +++ | 15 (40.54) | 5 (13.51) | 5 (13.51) | - | 9 (24.32) | 14/15 (93.33) | 5/5 (100) | 4/5 (80) | |
| P-value | P = 0.004 | P = 0.007 | P = 0.018 | P = 0.679 | P = 0.042 | P = 0.026 | |||
| Healthy (9) | |||||||||
| 0 | 2 (22.22) | 7 (77.77) | |||||||
| + | 7 (77.77) | 2 (22.2) | |||||||
Non-cancer = noncancerous tissues. 0 = no positive cell, + = positive in few areas or cells, ++ = moderately positive and +++ = predominately positive. ICC samples, comprising 37 matched cancerous and adjacent noncancerous tissues and 9 healthy subjects who died from accidental cases were collected at the time of surgery. ICC patients included 26 men and 11 women with the mean age 53±11 years. These subjects were verified by histopathological study and comprised well- (15 patients, including 2 patients of papillary), moderately- (11 patients) and poorly (11 patients) differentiated adenocarcinoma. The International Union Against Cancer TNM classification and staging system were used for tumor assessment.