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Expression of Nkx2.2 in parental (wt-ES cells) and Nkx2.2-transfected ES cells (Nkx-ES cells). A: Nkx-ES cells, but not wt-ES cells, expressed Nkx2.2 mRNA; B: Nkx2.2 protein was mainly observed in the nuclei of Nkx-ES cells. Original magnification, ×200.
