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Gene expression and immunohistochemistry findings for differentiating EB outgrowths. A: The expression of Pdx 1, Nkx2.2, pro-insulin 1 and pro-insulin 2 was examined in differentiating wt-ES cells and Nkx-ES cells; B: Insulin-immunoreactivity was examined in the EB outgrowths with DTZ-staining. Scale bars represent 100 µm in length.
