Figure 3.

Northern blot analysis for validation of PSTVd replication. Total RNA (2 µg) was extracted from PSTVd-infected and non-infected plants and subjected to long and small RNA analysis using the full-length PSTVd cDNA as a hybridization probe. Actin and U6 hybridization of the stripped membranes served as loading controls. Hybridization took place at 64°C and the blot was exposed for 12 hrs to phosporimager screen. At these hybridization conditions, PSTVd probe did not hybridize with GFP98(+) or GFPint98(+)/(−).