Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Northern blot analysis for pre-mRNA processing and degradation. Total RNA (7 µg) was extracted from PSTVd-infected and non-infected plants and subjected to long and small RNA analysis using the full-length GFP cDNA as a hybridization probe. Hybridization took place at 64°C and the blot was exposed for 24 hrs to phosporimager screen. Actin and U6 hybridization of the stripped membranes served as loading controls.
