Table 1.

Frequency of CYP9K1 haplotypes in pre‐ and post‐2006 individuals. To rule out the possibility of introgression of the cyp‐l haplotype from Anopheles gambiae, we genotyped 5 informative SNPs that span CYP9K1 and Tailless (+9.4 kb) to assess the frequency of the cyp‐l haplotype in Anopheles coluzzii and A. gambiae pre‐ and post‐2006. Note that cyp‐l was common in A. coluzzii (4–23%) and not detected in A. gambiae prior to the 2006 hybridization event. Also, cyp‐ll was more common that cyp‐l pre‐2006

	Frequency of CYP9K1 haplotypes, %
	A. coluzzii				A. gambiae
	2002	2004	2010	2014	2002	2004	2010	2014
cyp‐l	4	23	85	99	0	0	0	9
cyp‐ll	46	31	4	<1	0	0	0	0
cyp‐lll	48	39	12	0	100	97	100	87
cyp‐other	2	7	0	<1	0	3	0	4
N	26	35	26	158	13	18	21	21