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. Author manuscript; available in PMC: 2016 Jan 28.
Published in final edited form as: Cancer Lett. 2014 Oct 7;356(2 0 0):506–516. doi: 10.1016/j.canlet.2014.09.034

Figure 3. miR-21 completely disrupted clones derived from clone #38 using TALEN L2-R2.

Figure 3

(A) A homologous recombination event between the single-stranded oligodeoxynucleotides (ssODN) and the miR-21 genomic locus will delete the miR-21 sequence and replace with 3 bases of atc to generate a BamHI site. (B) Genomic DNA from clone #38124 was subjected to PCR and BamHI digestion (left) and Sanger sequencing (right). (C) Mature miR-21 levels were measured by RT-qPCR. (D) A heatmap of the miR-Seq data from clones #38 and #3837. The 49 most abundant microRNAs plus miR-21-3p were ranked and clustered by centering on the row-wise mean log(2) value [15, 17]. A scale of 8-fold deviation from the mean is shown.