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Transcript stability of selected genes in the WT and quadruple RNA helicase mutant L. monocytogenes strains. The stability of hly, prfA, SreB and tmRNA transcripts, respectively, was analyzed by Northern blotting of RNA isolated from wild-type (EGDe) or quadruple mutant (Δ4) strains harvested at indicated time points (in minutes) after rifampicin addition. Radioactively labeled DNA-probes specifically recognizing hly, prfA, SreB and tmRNA transcripts were used for detection.
