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. 2015 Jul 7;12(8):838–846. doi: 10.1080/15476286.2015.1058477

Figure 3.

Figure 3.

Pre-60S r-particles containing 7S pre-rRNAs engage in translation in the dob1–1 mutant. Wild-type (MTR4) and dob1–1 (mtr4) cells were grown at 30°C in YPD medium to mid-log phase. (A) Cells extracts were prepared and 10 A260 units of each extract were resolved in 7–50% sucrose gradients and fractionated. RNA was extracted from each fraction and analyzed by northern blotting using probes f, e and 5S, which reveal 7S pre-rRNAs and mature 5.8S and 5S rRNAs, respectively. The position of free 40S and 60S r-subunits, 80S ribosomes and polysomes, obtained from the recorded A254 profiles, are shown. (B) Signal intensities of the 7S pre-rRNAs (black dot, continuous line) and 5.8SS rRNA (white squares, dashed line) were determined for each fraction by phosphorimager scanning and represented as arbitrary units.