Skip to main content
. Author manuscript; available in PMC: 2016 Oct 12.
Published in final edited form as: Dev Cell. 2015 Oct 12;35(1):49–62. doi: 10.1016/j.devcel.2015.09.009

Figure 5. Young and old progenitors display differential adhesion properties when cultured in vitro.

Figure 5

(A-D) A 1:1 mixture of FACS sorted E12.5 and P0 Six2+ cells imaged live with a fluorescent stereoscope immediately after plating on top of a filter (A) and 48hr culture with heparin, FGF9 and BMP7 (B-D). (E-H) High magnification image of fixed heterochronic co-cultures. Antibody staining of Six2 marked in green. Note that the initial culture was a monolayer of evenly distributed cells (E-E’”). By the end of 48hrs, all cells formed aggregates (F-F’” to H-H’”). Scale bar, 30μm.