Transformed respiratory frequency (TRF) from WTLI (14.6 Gy) and representative sham-irrradiated (0 Gy) groups. Error bar represents the standard error in each study group. Based on the TRF, the data can be reviewed by two time periods, weeks 0–16 and weeks 18–26. The graph shows that the TRF for the WTLI-alone group is higher than for the WTLI and treatment groups (vehicle, 5, 10, 25 and 40 mg/kg) except at week 16 and 26. However, statistical analysis of the TRF in all groups reveals that in the first period of weeks 0–16, there is no statistical significance in most time points between the WTLI and treatment (vehicle, 5, 10, 25 and 40 mg/kg) and nontreatment, except at week 4, 8 and 10. From weeks 18–26, there is no statistical significance between WTLI and treatment (vehicle, 5, 10, 25 and 40 mg/kg) and nontreatment, except at week 22 where a significant difference is observed between the WTLI-alone and WTLI-vehicle groups. The lack of statistical significance from weeks 18–26 is due to greater standard errors observed in TRF, which are attributed to the lower number of live mice and larger variation in frequency.