Fig 3. CHIKVmos induces lower antiviral responses than CHIKVvero.
Raw 264.7 cells, L929 cells, NIH3T3 cells, and mouse bone marrow derived dendritic cells (mBMDC) were infected with CHIKVvero or CHIKVmos (Ross strain, MOI = 1) for 24 h. Gene expressions of Tlr3, Rig-I, Mda-5, Il-1β, and type I IFNs (Ifn-α and Ifn-β) were analyzed by RT-qPCR. The gene copy numbers were normalized to cellular β-actin and the data are presented as relative fold changes in expression of respective gene compared to the mock-infected control designated as 1 (not indicated in figures). All data sets represents two independent experiments performed in triplicates with similar results.